p beclin Search Results


p becn1 ser234  (Novus Biologicals)
91
Novus Biologicals p becn1 ser234
P Becn1 Ser234, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p becn1 ser234/product/Novus Biologicals
Average 91 stars, based on 1 article reviews
p becn1 ser234 - by Bioz Stars, 2026-02
91/100 stars
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p-beclin-1 thr119 antibody  (Merck KGaA)
90
Merck KGaA p-beclin-1 thr119 antibody
PKM2 increases the phosphorylation of Beclin-1 in leukemic cells. A The gene expression of Beclin-1 was determined by real-time PCR in OCI-AML3 cells following PKM2 knockdown. B The phosphorylation levels of Beclin-1 were assessed by western blot in PKM2-silenced OCI-AML3 cells. C-D The expression of p-Beclin-1 <t>Thr119</t> , Beclin-1, and PKM2 proteins were determined by western blot in THP-1 and NB4 cells after PKM2 overexpression. E-F The protein expression was assessed from the THP-1 and NB4 cells transfected with the plasmids expressing PKM2 WT or PKM2 K367M. The data are expressed as the mean ± SD (n = 3). * p < 0.05.
P Beclin 1 Thr119 Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p-beclin-1 thr119 antibody/product/Merck KGaA
Average 90 stars, based on 1 article reviews
p-beclin-1 thr119 antibody - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


PKM2 increases the phosphorylation of Beclin-1 in leukemic cells. A The gene expression of Beclin-1 was determined by real-time PCR in OCI-AML3 cells following PKM2 knockdown. B The phosphorylation levels of Beclin-1 were assessed by western blot in PKM2-silenced OCI-AML3 cells. C-D The expression of p-Beclin-1 Thr119 , Beclin-1, and PKM2 proteins were determined by western blot in THP-1 and NB4 cells after PKM2 overexpression. E-F The protein expression was assessed from the THP-1 and NB4 cells transfected with the plasmids expressing PKM2 WT or PKM2 K367M. The data are expressed as the mean ± SD (n = 3). * p < 0.05.

Journal: International Journal of Biological Sciences

Article Title: Glycolytic Enzyme PKM2 Mediates Autophagic Activation to Promote Cell Survival in NPM1-Mutated Leukemia

doi: 10.7150/ijbs.30290

Figure Lengend Snippet: PKM2 increases the phosphorylation of Beclin-1 in leukemic cells. A The gene expression of Beclin-1 was determined by real-time PCR in OCI-AML3 cells following PKM2 knockdown. B The phosphorylation levels of Beclin-1 were assessed by western blot in PKM2-silenced OCI-AML3 cells. C-D The expression of p-Beclin-1 Thr119 , Beclin-1, and PKM2 proteins were determined by western blot in THP-1 and NB4 cells after PKM2 overexpression. E-F The protein expression was assessed from the THP-1 and NB4 cells transfected with the plasmids expressing PKM2 WT or PKM2 K367M. The data are expressed as the mean ± SD (n = 3). * p < 0.05.

Article Snippet: Primary antibody as following: rabbit monoclonal antibody PKM2 (Cell Signaling, MA, USA ), rabbit monoclonal antibody LC3 І/II (Novus, Co, USA), rabbit monoclonal antibody p62 (Abcam, Cambridge, UK), Beclin-1 (Cell Signaling, MA, USA ), p-Beclin-1 Thr119 (Merck KGaA, Darmstadt, Germany), PTBP1 (Abcam, Cambridge, UK), PKM1 (Cell Signaling, MA, USA ), and rabbit polyclonal antibody β-actin (Proteintech, IL, USA) as loading control.

Techniques: Phospho-proteomics, Gene Expression, Real-time Polymerase Chain Reaction, Knockdown, Western Blot, Expressing, Over Expression, Transfection